Analysis Note

ControlLiver tissue.

Application

Anti-Cytochrome P450 Enzyme CYP2E1 Antibody is an antibody against Cytochrome P450 Enzyme CYP2E1 for use in IH & WB.

Immunohistochemistry:
A previous lot was used on formaldehyde treated and frozen sections.

Optimal working dilutions must be determined by end user.

General description

Cytochrome P450 (CYP) is a huge and diverse superfamily of hemoproteins. It metabolize the fatty acid arachidonic acid (AA) to regulators of vascular tone. Most CYPs can metabolize multiple substrates, and can catalyze multiple reactions. This accounts for their central importance in metabolizing the extremely large number of endogenous and exogenous molecules. In the liver, these substrates include drugs and toxic compounds as well as metabolic products such as bilirubin (a breakdown product of hemoglobin). Cytochrome P450 enzymes are present in many other tissues of the body including the mucosa of the gastrointestinal tract, and play important roles in hormone synthesis and breakdown (including estrogen and testosterone synthesis and metabolism), cholesterol synthesis, and vitamin D metabolism. More than 7700 distinct CYP sequences are known as of September 2007.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Rabbit polyclonal antiserum in buffer containing no preservative.

Quality

Evaluated by Western Blot on Human brain lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected Cytochrome P450 CYP2E1 on 10 µg of Human brain lysates.

Specificity

Reacts with human and rat cytochrome P450 enzyme CYP2E1 in hepatic microsomal fractions. No cross-reactivity with other cytochrome P450 enzymes in these species.

Target description

57 kDa